FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305868

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305868
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22955473
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG147200.044.60529794
CAAGTCG113350.026.08116794
TAAGTCG35550.023.00422394
GAAGTCG131700.019.66363194
AAAGTCG57600.013.70833494
CGCGAAT11600.012.9655181
GTTTATT107300.012.0018641
CGAAAAT36900.011.845531
CTTTATT154850.011.8372621
CGAAGAT90650.011.4583571
GGAAAAT177650.011.2175631
ATATACG10350.010.8985516
CGGAAAT42950.010.8335281
CGCGTAT4357.519741E-510.8045991
CTCGGAT66250.010.7833971
TATACGC8802.910383E-1110.6818197
GTTTAAT80700.010.4832721
CTCAGAT188450.010.4499861
GTCGGTT41650.010.3817531
AAATCGA35400.010.2231654
Produced by FastQC (version 0.11.5)