FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305871

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305871
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22681871
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG131950.059.4846694
CAAGTCG99750.028.93032694
GAAGTCG138450.024.44203894
TAAGTCG40550.022.02219494
AAAGTCG54700.015.55210394
CGAAAAT35750.015.5132871
TTTTTTC413750.014.0290032
GTTTTTT299100.013.781011
CGAATCG18050.013.0193913
GCCGAAT50000.012.4081
CTCGATT38000.012.3684221
GCGAAAT27900.012.1290321
AAATCGA34450.011.8693764
TACGCTA10050.011.6915439
TTTTTTG436700.011.6558282
AAATCCA204600.011.6466274
CGCGAAC9050.011.4254151
TATACGC8651.8189894E-1211.4104057
GTTTTCT231050.011.3914741
GTTTTTC184450.011.39007951
Produced by FastQC (version 0.11.5)