FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305873

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305873
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22964890
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG101900.051.93523494
CAAGTCG87750.028.86951694
TAAGTCG24000.024.47916894
GAAGTCG125250.017.14890394
GTTCGAT31450.016.4387911
AAAGTCG44100.013.748394
AATCGTA19200.012.9739585
TTCGATT35000.012.891432
TATACGG20350.012.35626734-35
GTCGAAT14200.012.2464791
TAGCGAG26600.012.01503859
TTAGCGA22450.011.9331868
GTCCGAT15500.011.8258081
TCGATTC39600.011.5126273
GTCGTAT11450.011.493451
GTCGAAA31000.011.2193561
TCGGTTA14100.011.0000012
GTCGGTT48650.010.9167541
GTCGTTT19850.010.8916881
GTATACG20700.010.78502634-35
Produced by FastQC (version 0.11.5)