FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305876

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305876
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22329014
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG131800.045.46661894
TAAGTCG31500.028.64761794
CAAGTCG100000.026.83794
GAAGTCG128100.021.06010894
CGAAAAC31200.014.762821
GTTTTTT307050.014.2966941
CGAAAAT41800.013.0430631
CGAATCG14250.012.8631573
CGGAAAT42000.012.6452391
AAAGTCG53100.012.480226594
CGCAAAC46000.011.751
CTCGATT38500.011.71948051
GGAAAAC154700.011.42341
CGCAATT21850.011.4004571
AAAAATC204750.011.1330892
AAATCGA34500.011.0347824
CGAAGAT80250.010.834891
GTTTAAT87850.010.8070571
GTTTTTC164200.010.76248551
GTTTTAT154700.010.7246281
Produced by FastQC (version 0.11.5)