FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305879

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305879
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21260710
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG175650.067.964794
CAAGTCG132950.035.95261494
TAAGTCG41950.030.69844894
GAAGTCG178200.028.14197594
AAAGTCG61200.022.34803894
TATATCG9100.013.4285717
TATACGG16650.012.9849858
GCAAGTC122100.012.58722494
GTATACG19500.011.087187
TTACGAG31700.010.8233441
ACAAGTC123700.010.79062392-93
TACGATT14500.010.69655194
GTATAGA37850.010.5548218
ATAAGTC47450.010.49947492-93
TTTTCGA38750.010.3096777
CGAAGTC95700.09.94514192-93
CGTATCG22300.09.69506736-37
TATGAGT60950.09.4848235
ATATCGC15450.09.4304218
AGAAGTC237250.09.37028594
Produced by FastQC (version 0.11.5)