FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305880

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305880
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21260710
Sequences flagged as poor quality0
Sequence length100
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAT25300.016.3478261
GTTTTTT263100.014.1125051
CGCAAAC44100.014.06802751
CTCGATT33600.013.8482141
CGCAAAT24100.013.8464731
CGCGAAT13100.013.2748091
AAATCCA179700.012.9727334
AAAAATC188900.012.9629432
GGAAAAC139600.012.9620351
GCCGAAT46100.012.9479391
TCGATTG19750.012.8506342
GTTTAAT80800.012.6806941
AATCCAC108450.012.5246665
GGAAAAT163700.012.1160661
CGAAAAT47100.012.074311
CGTGATA20650.011.6077499
GAAAAAT193300.011.5980341
CGAAAAC31250.011.58081
AAATCGA28600.011.5034964
CGGATAT20550.011.2068121
Produced by FastQC (version 0.11.5)