FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305881

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305881
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22879464
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG106000.053.38490794
CAAGTCG84050.028.18322694
TAAGTCG31900.026.22570694
GAAGTCG124550.021.96226594
GTTCGAT28700.013.5923361
AAAGTCG46150.011.91549494
TTCGATT32300.011.0588242
TCGGATT25950.010.6859352
CGGAAAT43250.010.6497131
GTCGTTT20600.010.4951471
TATACGG17150.010.41399534-35
CTCGGAT63300.010.3206961
GTCCGAT15700.010.1783451
CGCGGAT25000.010.1519991
CGCGTTT12550.010.1115541
GTCGGAA35850.010.094841
GTCGAAT15550.09.6720261
GGGAAAT157600.09.5729691
AATCGTA22600.09.5663725
GTCGAAA34900.09.56160451
Produced by FastQC (version 0.11.5)