FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305886

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305886
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22951992
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG115900.041.484994
CAAGTCG99800.025.94889694
TAAGTCG29400.025.5782394
GAAGTCG111900.016.50670294
AATCGTA18100.015.580115
CGCGTAT5157.8216544E-1114.6019411
AAAGTCG48550.013.35942294
CGCGTAA5909.156793E-911.9491521
TTAGCGA19400.011.3865988
CGAATCG16200.011.0246913
TAGCGAG23450.010.8230289
CGGAAAT39550.010.6953221
CGTATCG15100.010.5827813
CGGGAAT53250.010.5032861
GTCGGTT47350.010.3231251
GTTCGAT25450.010.1571711
GTCCGAT14000.010.0714281
CGCGGAT26800.09.9962681
TACCGTA18200.09.8131879
GGCGAAT32350.09.5888711
Produced by FastQC (version 0.11.5)