FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305890

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305890
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22330628
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG143850.048.0945494
CAAGTCG108850.028.32521894
GAAGTCG127800.021.3669894
TAAGTCG34300.021.23906794
GTTTTTT312800.014.9354221
GCGAAAT33800.014.8786981
AAAGTCG56800.014.14964994
CGCATTC41700.012.8489211
CGAAAAC28100.012.7117431
CGAATCG16500.012.2484853
CGTATCG19500.012.05128250-51
TTAGCGA24650.012.0121717
CGCAATC26650.011.9924961
CGAAAAT53100.011.9491521
CTTTAAC87400.011.884441
AAAAATC214450.011.8568422
CGCAAAC43500.011.7770111
AAATCCA201100.011.7324714
CCCGAAC48200.011.7012441
CGCAAAT27750.011.6864861
Produced by FastQC (version 0.11.5)