FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305891

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305891
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21264282
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG171700.063.67035794
CAAGTCG130750.033.17858594
TAAGTCG42500.033.1764794
GAAGTCG173950.027.61368294
AAAGTCG58050.023.15590194
TATACGG15700.014.6687918
GTATACG16950.013.8643077
TTACGAG32400.012.9104961
CGCGTAA7150.012.1608488-89
GCAAGTC127450.011.5056994
ACAAGTC125000.010.86640294
TCGCGTA8850.010.621469586-87
CGAAGTC83200.010.50721392-93
TTAAGTC65350.09.92501994
AGAAGTC237500.09.7562194
ATAAGTC50350.09.75471892-93
GCGTATA10508.185452E-119.4000016
TAGCGTA9852.4556357E-99.065994
TACGAGT44100.09.0589572
CGTATCG20650.08.99031536-37
Produced by FastQC (version 0.11.5)