FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305893

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305893
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22024514
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG126850.056.09617694
TAAGTCG35900.028.14763394
CAAGTCG98300.026.67955494
GAAGTCG141350.020.881594
AAAGTCG49550.016.03027394
GTTTTTT284350.014.1983471
TTTTTTC351300.013.124682
CGAAAAT32300.012.222911
GTTTAAT75000.012.221
CGGAAAT41500.012.0048191
GCCGAAT46800.011.5491451
GGAAAAT167550.011.5290961
AAAAATC186800.011.5235562
GTTTTTC156650.011.5212251
CGCGAAC14350.011.4634151
AAATCCA186200.011.2830294
TTTTTTG406450.011.2513232
CGCAAAT31400.011.2261151
GTTTTAC64200.011.2009341
AAATCGA28500.011.0491234
Produced by FastQC (version 0.11.5)