FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305894

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305894
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22024514
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG150600.047.094
CAAGTCG113600.025.44454494
TAAGTCG35500.023.96337994
GAAGTCG138250.020.15985794
AAAGTCG57250.016.66550394
GTTTTTT272000.013.0805151
CGGAAAT40950.012.7399271
CGAAAAC32500.012.7261531
CGAAAAT36800.012.6440231
CTCGATT31550.011.9175921
CGCGAAT8401.8189894E-1211.751
GCCGATT20200.011.6336641
GTTTAAT76950.011.482781
CGCAAAC45600.011.337721
AAATCGA29850.011.0217754
GTTTTCT208950.010.9318021
AAATCCA183700.010.9248784
CGCGTTT17550.010.7122511
AAAAATC178650.010.6549122
GGAAAAT163050.010.55013751
Produced by FastQC (version 0.11.5)