FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305897

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305897
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23681869
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG140750.059.00462394
CAAGTCG101150.031.2713894
GAAGTCG154450.021.51440694
TAAGTCG44100.021.10204194
AAAGTCG57150.016.61242394
TACGCTA7600.014.8421069
CGCGAAC16800.013.7083341
CTTTATT214550.013.6695411
GTCGGTT49050.012.3608571
CGAAGAT97500.012.3405131
CGAAAAT32400.012.3302471
AATCGTA25300.012.260875
GTTTATT117750.012.2539281
GGAAAAT201950.011.7994561
TATACGC9600.011.757
TTTTTTC394800.011.7380962
GTTTTTT322150.011.7007611
AAATCCA199350.011.4818174
ATATACG11900.011.4537826
CGCAAAT31350.011.24401951
Produced by FastQC (version 0.11.5)