FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305898

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305898
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23681869
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG166350.049.75473494
CAAGTCG123200.027.81087994
TAAGTCG47000.021.70000394
GAAGTCG146500.020.08327794
AAAGTCG60900.016.05254694
CGAATCG18150.013.9834723
GTTTATT117700.012.378931
CGAAGAT95450.012.3593511
GGCGAAT39550.011.8836921
GGAAAAC162300.011.757241
CTTTATT204300.011.7557521
AATCGTA22600.011.6460175
CTCAGAT179550.011.5176841
CGCGTAT4901.7218917E-611.5102041
GGAAAAT192300.011.4872611
GCGGTAT22100.011.2714941
CGGAAAT44000.011.2159091
GTTTTTT301100.010.9734321
GTTTAAT80950.010.8573211
CGCAAAT31400.010.777071
Produced by FastQC (version 0.11.5)