FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305901

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305901
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22740720
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG97650.050.29697894
CAAGTCG77350.025.27731194
TAAGTCG26600.022.26315794
GAAGTCG105950.016.3246894
CGCGTAT4802.5465852E-1115.6666671
AAAGTCG44550.013.50392894
GCGGTAT21900.013.3059361
AATCGTA21000.011.4142865
GTCGGTT42250.011.0130181
ATCGTAT17150.010.9620996
GTCCGAT15100.010.894041
TAATCGT20250.010.4444444
CGGGAAT51200.09.91406251
CGCGAAT11900.09.8739491
GTCGCAT21350.09.6861831
GTTCGAT26300.09.650191
CGGTATA14400.09.4652782
CGCGGAT23350.09.4603861
GCGGGTT42850.09.3232211
GTCGCTT30600.09.2156871
Produced by FastQC (version 0.11.5)