FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305902

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305902
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22740720
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG116550.042.4633294
CAAGTCG95650.023.14375394
TAAGTCG26600.021.5563994
GAAGTCG101700.016.1288194
CGCGTAA5754.474714E-1013.078261
GCGGTAT19250.012.4519481
AATCGTA19950.011.54385955
ATCGTAT16400.011.4634146
AAAGTCG47600.010.56512594
TAATCGT19000.010.3894734
TTAGCGA18050.010.1551268
GGGAAAT157050.010.1451771
GTTCGAT25500.09.9529411
CGGAAAT39900.09.6591481
GCCGGTT46750.09.6513381
GGCGATT36250.09.5944821
TAGCGAG21550.09.378199
CGGGAAT49700.09.3621731
TCGGATT25550.09.1976522
CGTAACG4700.00182544859.0000013
Produced by FastQC (version 0.11.5)