FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305903

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305903
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22285275
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG139400.061.29555594
CAAGTCG120000.033.29166894
TAAGTCG35250.028.13333594
GAAGTCG142150.021.62363894
AAAGTCG47350.019.55438294
TTAGCGA20750.012.4578318
GTTCGAT24500.012.0857151
GTCGGTT47400.011.50211051
CGTATCG16350.011.211013
GTCGGAT26500.011.1735861
GTCGAAA36400.011.1043961
GTCGGGT43400.010.7211991
CGCGGGT67150.010.7088611
TAGCGAG25650.010.6276819
GGCGAAT63950.010.5832691
GGGAAAT154700.010.542341
CTCGGTT33500.010.5223881
GTCGGTC43950.010.3731521
TATACGG13900.010.3129534-35
GTCGAAT11900.010.26890851
Produced by FastQC (version 0.11.5)