FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305904

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305904
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22285275
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG158600.053.6677294
CAAGTCG138000.031.36739294
TAAGTCG35100.027.31623894
GAAGTCG140100.021.00071594
AAAGTCG48600.017.02057894
GTTCGAT24300.013.345681
GTCCGAT12600.011.9365081
AATCGTA17800.011.8820235
CGGGAAT50550.011.157271
CGCGGAT25350.010.9388571
GCCGGTT48800.010.7868861
GGCGATT40600.010.7660111
TTAGCGA20000.010.5758
TTCGATT28700.010.4808372
CGCGGTT39600.010.4444451
TACCGTA20950.010.319819
GGGAAAT146850.010.3057551
GTCGTTT20100.010.2885581
GTCGGAT35950.010.19749694
GTCGGTT42050.09.8359111
Produced by FastQC (version 0.11.5)