FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305905

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305905
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22355274
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG114300.057.1977394
CAAGTCG87750.027.2626894
TAAGTCG36150.025.35269794
GAAGTCG126350.021.94697494
AAAGTCG50100.015.66666894
GTTTTTT316900.015.0536451
CGCGAAC12950.014.5173761
CGAAAAT37900.013.6411621
GTTTAAT99100.012.9949551
GTCGAAT15550.012.6945341
GTTTTCT250850.012.2348011
TTTTTTC413000.012.2336572
AAAAATC240100.011.7451072
GTCGATT20900.011.693781
GTCGAAC44300.011.6704291
GTTTTAT173800.011.6553521
GGAAAAT188250.011.3598951
CGAATCG14600.011.2671243
GAAAAAT245750.011.2455751
GTTTTTC198350.011.1131841
Produced by FastQC (version 0.11.5)