FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305906

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305906
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22355274
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG134650.048.06461394
CAAGTCG106900.025.58840294
TAAGTCG36050.025.553494
GAAGTCG126650.020.52191294
CGCGAAC13550.014.915131
GTTTTTT321750.013.848021
CGAAAAT40450.013.1297911
AAATCGA36250.012.7062074
AAAGTCG54400.012.70036894
GGAAAAT193100.012.0968411
CGCAAAT30700.011.9413681
GTTTAAT95500.011.811521
CGGATAT19350.011.65891551
CTCGGAT58150.011.6388651
TATACGC8401.0913936E-1111.1904767
TCGAATC20200.011.1683182
CGCGGAT26250.011.1009521
CTCAGAT168650.010.92441
GTTTTCT246650.010.8615451
TACGCTA7402.040906E-910.79729759
Produced by FastQC (version 0.11.5)