FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305907

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305907
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22944060
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG105500.056.5336594
CAAGTCG86200.029.66125394
TAAGTCG31550.023.98415494
GAAGTCG126900.019.2592694
AAAGTCG46000.014.71304494
CGCGTAA7750.014.554841
GTTCGAT29200.014.3253431
TTCGATT33300.011.9969972
CGCGTAT5002.6997228E-510.341
TCGGATT26550.010.267422
CGCGAAT14700.010.2312941
TATACGG18050.010.15512634-35
GTCGATT21150.010.01
GTCGGTT52000.09.76153851
TACCGTA18000.09.6611129
CTCGGAT61600.09.6136371
TAGCGAG25850.09.4545459
TTAGCGA22500.09.4000018
GTCGAAA34050.09.3861971
GGCGAAT56500.09.3168141
Produced by FastQC (version 0.11.5)