FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305908

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305908
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22944060
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG134000.045.10597294
CAAGTCG98050.026.22029994
TAAGTCG30100.023.89036894
GAAGTCG133250.017.28330294
GTCCGAT16050.012.2990671
AAAGTCG57950.012.16566194
GGCGAAT48000.011.5541671
CTCGGTT39850.011.0865751
TTAGCGA21700.011.0460838
TCGGATT25850.010.9090912
GGCGATT40700.010.8550371
CGGGAAT56500.010.5646021
CTCGGAT59450.010.4356611
GTTCGAT30750.010.39349651
CGCGTTT15850.010.378551
CGCGGTT44850.010.2697891
AATCGTA19850.09.9445845
GGGAAAT160700.09.797761
GGAAAAT186250.09.5892621
CGCGTAA6454.4036096E-69.4728691
Produced by FastQC (version 0.11.5)