FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305909

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305909
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22877728
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG118300.054.78698394
CAAGTCG103100.030.81668394
TAAGTCG33900.025.64896894
GAAGTCG122350.019.32243594
CGCGGAT25250.013.0297031
AAAGTCG49250.012.78781794
GTTCGAT34250.012.4875921
GTCCGAT14700.012.4693881
CGCGTAA9800.012.4693881
GTCGAAT14750.012.4271191
CGCGTTT15200.012.059211
GTCGGAT32400.011.751
TTCGATT35350.011.4342292
GTCGGTT44200.010.9524891
AATCGTA18750.010.77866655
GGGAAAT152550.010.35201551
TTAGCGA20000.010.348
TAGCGAG24750.010.2545449
GTCGAAA33150.010.2081441
GTCGTAT11750.010.01
Produced by FastQC (version 0.11.5)