FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305910

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305910
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22877728
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG143350.045.08196394
CAAGTCG113100.028.09195594
TAAGTCG35200.024.0340994
GAAGTCG127550.018.97687194
GTCCGAT16750.012.6268651
GTCGTTT24650.012.3935091
GCGGTAT28700.012.1184671
TATACGC7703.3651304E-1010.9870147
AAAGTCG55750.010.87533694
GTCGTAT11850.010.3122361
GTCGGTT39750.010.2867921
CGTATCG18100.010.1270723
GGCGATT40550.010.0838471
CGGGAAT54250.010.0497691
CGCGTAA10407.2759576E-129.9423071
TTAGCGA20900.09.8947378
TAGCGAG27550.09.7241389
CGACCTA12100.09.7107443
GGGAAAT144800.09.5428181
GCCGGTT55050.09.3914621
Produced by FastQC (version 0.11.5)