FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305911

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305911
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22787778
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG130150.058.8989694
TAAGTCG35900.030.50417594
CAAGTCG103700.029.64127294
GAAGTCG137500.022.86763694
AAAGTCG51300.017.6822694
CGAAAAT36150.015.8616871
GCGAAAT27350.015.2943321
CGCAATT19100.015.2565441
GTTTTTT307250.015.2052071
GTCGAAT14100.013.9999991
AAAAATC224150.013.6502332
GAAAAAT226050.013.3691661
AAATCCA216100.013.0060154
GTTTAAT90900.012.7711771
TTTTTTC396200.012.7168092
GTTTTTC178650.012.4965011
GCCGAAT48750.012.2441021
AATCCAC126700.012.2415145
CTCGATT36450.011.9917691
GTCGATT23150.011.9784021
Produced by FastQC (version 0.11.5)