FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305917

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305917
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21587245
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG132000.061.27802794
CAAGTCG110200.033.18148894
TAAGTCG33650.029.47102794
GAAGTCG150950.022.10665794
AAAGTCG47050.016.68225394
CGCGTAT5653.3833203E-1013.3097341
TATACGG15900.011.82389934-35
GTTCGAT32750.011.7679391
CGCGGGT80000.011.691251
CGGGTCT114300.011.1023633
GTCGAAT12900.010.9302321
ATCGTAT13450.010.8327146
CGTATCG16450.010.5714293
TTCGATT34750.010.549642
GGGAAAT147300.010.274271
GTCGAAA31400.010.1783441
TCGGATT26700.010.0337082
GCGGTAT23900.010.0292881
GGCGGTT53800.09.9591081
GTCGGAT30100.09.8372091
Produced by FastQC (version 0.11.5)