FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305918

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305918
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21587245
Sequences flagged as poor quality0
Sequence length100
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG152200.052.71287594
CAAGTCG126050.033.2225394
TAAGTCG33400.026.45508894
GAAGTCG138200.021.22141894
AAAGTCG53150.015.12135494
CGCGTAT4253.0761294E-713.2705891
TTAGCGA19500.012.2923088
GTCCGAT12850.011.7042811
TAGCGAG24250.011.6288669
GTCGAAT12350.011.4170051
GGCGATT38850.010.7670541
GTCGAAA31150.010.7126811
GCGGTAT25100.010.2988051
AATCGTA19650.010.2849875
CGCGGAT26450.09.95085051
ACAAGTC116150.09.83297594
GTCGTTT18900.09.6984131
GTTCGAT35600.09.637641
CCGGTTT34800.09.5890811
GGCGGAT88400.09.5169681
Produced by FastQC (version 0.11.5)