FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305919

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305919
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22207067
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG101950.047.6684794
CAAGTCG76950.027.24106894
TAAGTCG29600.023.18243494
GAAGTCG118700.018.68913394
CGAGAAC73500.017.0734711
GCGGTAT25300.014.3043491
TACGCTA8200.013.1829279
AAAGTCG56400.013.08333394
CGCGAAT11850.011.8987351
CGAAGAT89500.011.8156421
GTTTTTT297900.011.5961731
AATCGGG41250.011.5078795
CGAATCG15350.011.3289913
TCGGATT35850.011.2747562
CTCGTTT37050.011.1632931
GCGGAAT40250.011.0931681
CTCGTAT16800.010.9107151
CGAGAAT41050.010.876981
GGAAAAT199900.010.838921
GTTTTAT158600.010.8165191
Produced by FastQC (version 0.11.5)