FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305920

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305920
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22207067
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG118950.037.1416694
CAAGTCG90800.024.06938694
TAAGTCG34750.021.23453394
GAAGTCG109350.017.27846394
CGAGAAC72350.016.1755351
CGAATCG16350.014.08562853
CGAAGAT83250.012.8720731
AATCGTA21800.011.4266065
CGGAAAT52050.011.3775221
CGCAAAC49150.011.2838261
GGCGAAT35950.011.2433941
CGGACAT57850.011.1305091
GTCGATT16100.011.0931681
CTTTAAT119050.011.0541791
GTCGGTT38150.010.9646141
TCGCAAA40800.010.94362814-15
AAAGTCG60250.010.92116394
CGCGTTC26800.010.8731351
CGCAAAT32050.010.8517941
AATCGGG34950.010.7582265
Produced by FastQC (version 0.11.5)