FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305923

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305923
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22558378
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG118700.054.0480294
CAAGTCG95050.027.0478794
GAAGTCG128150.022.48224694
TAAGTCG29600.021.4358194
GTTTTTT281550.016.3761311
AAAGTCG52400.016.32442794
TTTTTTC379900.015.1181892
CGAAAAT37250.014.888591
GCGAAAT28750.014.8765211
GCCGAAT53750.013.9032551
GAAAAAT212200.013.4000941
CGCAAAT29500.012.9050841
GTTTTAC63200.012.8655061
TTTTTTG396700.012.8311062
TATACGC9000.012.5333337
GGAAAAT162400.012.4735221
TACGCTA8350.012.3832339
CTCGATT36900.012.3550141
AAATCCA192350.012.2906164
TCGGATC42100.012.2802852
Produced by FastQC (version 0.11.5)