FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305924

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305924
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22558378
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG145600.044.5144294
CAAGTCG109650.025.50387694
TAAGTCG33700.020.50148294
GAAGTCG133050.020.09996294
GCGAAAT28650.015.9127391
CTCGATT35800.014.966481
CGGATAT21350.014.3091331
CGAAAAC34900.014.0057311
GCCGAAT48600.013.9259261
GTTTTTT282550.013.9228451
CGAAAAT39850.013.5633621
CGCGAAT7950.013.0062891
AAAGTCG60350.013.00579994
CGCAAAC39700.012.3123421
AAATCCA184700.012.2907414
GGAAAAT165450.012.2435781
CCCGAAC50050.012.2077911
GTTTAAT79300.012.0907941
AAAAATC200800.012.0776882
CGGAAAT47100.011.9745221
Produced by FastQC (version 0.11.5)