FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305926

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305926
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23000138
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG132900.042.93303394
CAAGTCG100350.022.90284294
TAAGTCG32500.022.12615494
GAAGTCG127750.018.8735894
GTTCGAT30700.017.4527681
AATCGTA20750.016.7614465
TTCGATT34400.014.345932
CGCGTAA6950.013.525181
TCGGATT28800.013.2187492
GTCCGAT14600.013.1986291
ATCGTAT18150.012.1707986
TCGATTC36100.011.7174523
CGTATCG18150.011.6528923
GTCGTTT22300.011.17040351
CGCATTT31000.011.0677421
AAAGTCG49950.010.91491694
TATACGG20550.010.86374834-35
CGGAAAT43100.010.46867751
CTCGGTT35400.010.2231651
CGCGATT6504.4513217E-710.1230761
Produced by FastQC (version 0.11.5)