FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305927

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305927
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22634771
Sequences flagged as poor quality0
Sequence length100
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG110400.053.38586894
CAAGTCG86650.028.74783594
TAAGTCG31900.025.34169294
GAAGTCG131050.019.90461594
CGCGTAT3753.785317E-916.2933331
AAAGTCG51000.016.21960694
TTTTTTC379150.015.3588282
GTTTTTT290300.014.9435061
TTTTTTG390100.014.6867472
GTCGATT21050.013.3966741
TTAGCGA21150.012.8888897
GTTTAAT86000.012.67906951
GCGGTAT30550.012.4615371
CGAAAAT32000.012.3374991
CGCGGAT22900.012.314411
AATCCAC111500.012.0556055
TTTTTTA362850.011.9297222
GGAAAAT164750.011.667981
AAAAATC193400.011.5191312
TATACGC11050.011.4841627
Produced by FastQC (version 0.11.5)