FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305929

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305929
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21185347
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG202150.056.56739894
CAAGTCG134700.032.20564394
TAAGTCG49400.030.8259194
GAAGTCG184150.026.7477694
AAAGTCG62700.022.48803994
TATACGG17800.012.1460688
GTATACG19100.011.8115187
ATAAGTC49450.011.31041494
GCAAGTC121200.010.7417594
ACAAGTC129450.010.710794
CGAAGTC86800.010.39631492-93
AGAAGTC250250.010.31088994
CGATAAT36900.010.1897024
TTACGAG27900.09.4336921
CGTATCG21750.09.40000136-37
TTAAGTC64900.09.08859792-93
GCGTATA8954.587673E-88.9273756
TAGAGCG34450.08.8679251
CTTCGAT45750.08.6295081
ACGGAAC45300.08.6114793
Produced by FastQC (version 0.11.5)