FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305930

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305930
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21185347
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTTTT263500.015.3396591
CTCGATT35350.014.8910891
AAATCGA30100.014.0531564
GCGAAAT27150.014.0220991
CGCAAAC41500.013.4771081
AATCGTA18850.013.2148545
CGAAAAT45850.013.1210461
AAATCCA182800.012.5213344
CGCAATC26050.012.4491371
GCCGAAT46600.012.4055791
CGAATCG16450.012.2857143
GTTTAAT85500.012.1485391
AAAAATC197450.012.1397832
CGCGTTT16350.012.0733941
CGGATAT21200.011.9716981
GGAAAAT155450.011.9125131
CGCAATT19850.011.8387911
GCGAATT13150.011.7946771
GTTTTAC65600.011.6783531
CGCAAAT25550.011.5890421
Produced by FastQC (version 0.11.5)