FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305931

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305931
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22516593
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG131700.058.3842194
CAAGTCG108000.027.24259294
TAAGTCG42350.025.85832494
GAAGTCG141750.025.06666694
GTTTTTT299500.016.6030041
AAAGTCG54650.016.16834494
CGAAAAT36250.015.4289651
TTTTTTC426700.014.9470352
TATACGC8050.014.5962727
CGCGAAT8900.014.2584261
CGAATCG19800.012.5808083
TTTTTTG434400.012.5614632
CGGATAT21800.012.5045871
AAATCGA33100.012.4954684
TCGAATC23550.012.37367252
GAAAAAT226700.012.2734891
GTTTTAC78300.012.0651331
AAAAATC221650.012.0018042
GGAAAAT190900.011.9654261
CGCAAAT31050.011.9581321
Produced by FastQC (version 0.11.5)