FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305932

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305932
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22516593
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG149650.051.72669294
CAAGTCG120300.027.5827194
GAAGTCG136700.023.1389994
TAAGTCG44250.021.7740194
CGCGAAT6900.019.0724621
AAAGTCG61900.016.47657494
GTTTTTT298250.015.2385581
TACGCTA8100.013.9259259
CGCAAAC43900.013.9179951
CGGAAAT41900.013.90930751
AAATCGA34850.013.3515064
GGAAAAT186600.013.2990351
CTCGATT33600.013.1488091
GTTTAAT90900.012.51265051
GCGAAAT27050.012.5101661
AATCGAC22650.012.450335
CGCAATT20800.012.4278831
CGAAAAC33100.012.3534741
CGAATCG16450.012.2857133
CGCAAAT31950.011.9154931
Produced by FastQC (version 0.11.5)