FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305933

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305933
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22884155
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG99800.047.1412894
TAAGTCG32200.022.77018594
CAAGTCG78150.022.07165594
GAAGTCG120750.016.69813594
CGCGGAT23400.012.45299151
GGAAAAT184800.012.3095231
AAAGTCG45450.012.2024294
CGAAAAT30850.012.0356561
GTCGGTT43900.011.9908891
TATACGC8350.011.8203587
CGGAAAT47550.011.6635121
GTTTTTT292850.011.0899771
GTTTTAC71700.010.9470011
TTTTTTC381100.010.9391242
GTTTAAT83150.010.90921
GTTTTAT148400.010.7998651
TTCGATC21350.010.7868842
TCGGATT24500.010.7428572
GTTTATT112200.010.7237071
GTCGAAT14050.010.7046261
Produced by FastQC (version 0.11.5)