FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305934

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305934
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22884155
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG125850.037.42073494
CAAGTCG96250.023.09714194
TAAGTCG31700.017.94006294
TATACGC8000.017.6249987
GAAGTCG114850.015.0187294
TACGCTA9550.014.7643989
CGAATCG16650.014.3963963
ATATACG11950.012.5857746
CGCAAAT30850.012.3403571
CGGAAAT42700.011.3372361
CGAAGAT80050.011.3316671
CAATCGA21050.011.1638964
CGCGATT8056.184564E-1111.0931671
AATCGAC21650.011.0715925
GGAAAAT179950.010.9697131
CGCGGAT27700.010.689531
CGCAAAC43250.010.649711
GTTTTAT141250.010.6477871
AAAGTCG54800.010.63503694
CTCGATT35950.010.5897071
Produced by FastQC (version 0.11.5)