FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305935

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305935
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22561626
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG100350.051.00448294
CAAGTCG83500.029.04431294
TAAGTCG28000.025.84999894
GAAGTCG113850.018.45322894
TTAGCGA22200.015.4549548
AAAGTCG41300.013.88377794
AATCGTA20450.013.7897315
GTTCGAT26250.013.7866671
TAGCGAG28750.013.7321739
TTCGATT31200.011.752
CGCGTTT12050.011.7012441
GCGGTAT22950.011.67320251
GTCGGAT26900.011.5315981
GTCGGAA38900.011.4781481
ATCGTAT16350.011.2110096
GCCGATT21850.011.1853541
GGCGAAT55850.011.1083261
GTCCGAT15750.010.7428581
GTCGAAT13700.010.6350361
CGCGTAT4459.470317E-510.5617981
Produced by FastQC (version 0.11.5)