FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305938

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305938
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22506747
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG143250.049.6083894
CAAGTCG116850.026.34574394
TAAGTCG37000.022.99189294
GAAGTCG131250.021.27085794
AAAGTCG57550.015.68027994
CGAAAAC33450.014.4723471
GCCGAAT46750.014.37647151
CTCGATT31000.013.4935481
AAATCGA36300.013.3360894
GTTTTTT286900.013.3021961
CGAAAAT37900.012.8970971
GGAAAAT182950.012.0229571
CGGAAAT38500.011.9636361
CGAATCG15150.011.7887783
AAATCCA201000.011.7383094
GCGGATT25650.011.7270961
GCCGATT21650.011.7228631
CGCGTTT16050.011.7133961
CTCGAAT39000.011.6897451
CGCAAAC41750.011.595211
Produced by FastQC (version 0.11.5)