FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305939

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305939
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21625666
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG120900.056.33002594
CAAGTCG89100.028.2738594
TAAGTCG36650.027.57162794
GAAGTCG141250.023.42513594
GTTTTTT275050.015.4815491
AAAGTCG50400.015.10714394
TTTTTTC365250.013.4598232
TATACGC9250.012.7027037
AAATCGA29500.012.5864424
AAAAATC201350.012.5815752
CGAAAAC30650.012.2675371
CGCGAAC16100.012.2608711
GAAAAAT203600.012.2347761
TACGCTA9650.012.1761679
AAATCCA186400.012.0525764
GGAAAAT172050.012.047081
GTTTTAT154600.011.9780081
CGAAAAT31800.011.9716981
GTTTAAT83500.011.6514981
GTTTTAC70000.011.5485721
Produced by FastQC (version 0.11.5)