FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305941

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305941
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22149214
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG106550.052.4917994
CAAGTCG82100.028.62362994
TAAGTCG33500.023.28955394
GAAGTCG120400.019.79152994
AAAGTCG48550.014.71472794
GTCGGTT44750.013.5486031
GTCGAAT14000.012.4214281
CGAAAAT36350.012.4126551
GCGGTAT25400.012.2125981
CGGAAAT49100.011.6782071
CGAATCG16400.011.4634153
CGAAGAT86050.011.4154561
AAATCCA203900.011.4100064
GTTTAAT92250.011.3615181
GGAAAAT173950.011.2129921
GTTTTAT148000.011.1148641
GAAAAAT224550.011.0932981
TTAGCGA22250.010.984277
GTTTATT107100.010.9710541
GTTTTTT279500.010.8293391
Produced by FastQC (version 0.11.5)