FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305943

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305943
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21811199
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG103200.053.7403194
CAAGTCG82550.029.60629794
TAAGTCG31700.023.27760194
GAAGTCG124100.020.4891294
AAAGTCG50400.018.3710394
GTTTTTT294250.014.3595571
TTTTTTC394950.014.2207882
GCGAAAT27700.014.0830311
CGAATCG16400.013.7560983
AATCCAC115350.013.2830515
GAAAAAT213350.012.7330671
TTTTTTG421250.012.4515132
GGAAAAT166300.012.1809981
AAATCCA192300.012.1716054
CGAAAAT36000.011.8805551
GTCGAAC44850.011.8416941
GTTTTAC66650.011.7059261
AAAAATC203900.011.5713582
GTCGATT19650.011.4809161
TTTTTTA396150.011.3421682
Produced by FastQC (version 0.11.5)