FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305944

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305944
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21811199
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG131700.042.18222894
CAAGTCG97900.025.82839694
TAAGTCG33600.025.3184594
GAAGTCG123900.019.83938694
TATACGC9200.013.2826087
GTTTTTT285300.013.2779521
CGCGAAC14950.012.8896311
CGAAAAT42000.012.7571421
CGCAAAC42000.012.7571421
AAAGTCG56800.012.66021194
CGAAAAC35400.012.2146891
GCGAAAT28500.012.0385961
CGGAAAT44750.011.7631271
GTTTAAT87250.011.6893971
CGGATAT23850.011.6268331
GGAAAAT167300.011.3777631
GCCGAAT46350.011.2556641
AAAAATC193900.011.1985552
GCGGTAT26800.010.8731341
CGAATCG15250.010.7868843
Produced by FastQC (version 0.11.5)