FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305945

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305945
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22002300
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG62450.036.124994
CAAGTCG45450.019.23432494
TAAGTCG18900.018.40211594
GTTTATT103250.014.8852291
CGCAAAC42450.014.3934031
GTTTTAC56550.013.13174251
GTTCGAT28250.012.9769921
GTCGGTT61450.012.9259561
CGAAAAT37900.012.2770441
GGAAAAT170650.012.22853851
CGGAAAT44600.012.1188341
CGCGTAT4701.041828E-612.0000011
CGCAAAT27050.011.988911
CTTTATT168550.011.9626231
GTTTTAT166600.011.9333731
GTTTAAT100400.011.8904381
GTTTTTT305400.011.6961361
GGAAAAC145750.011.6411671
GTAAAAC71900.011.6356061
GTTTGGT166600.011.42557051
Produced by FastQC (version 0.11.5)