FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305946

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305946
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22002300
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG80050.028.18238894
CAAGTCG63550.016.49252594
TAAGTCG19450.015.22365294
CGCAAAC44050.015.1509651
GTTCGAT33850.014.856721
GTTTATT110600.014.6609411
GTCGTTT19750.013.8025321
CGCGTAT4101.9858817E-713.7560981
GTTTTAT144700.013.1548021
GTAAAAC63050.012.821571
GGAAAAC138950.012.75206851
CTTTATT157350.011.8284081
GTCGGTT50550.011.6221571
GTCAACT98900.011.5480281
CGAATCG14550.011.3058423
GTTTTAC67350.011.3051221
GCGCGTT35550.011.30379892-93
GTCGGAT27650.011.0488251
GTTTTTT295300.010.9979681
TTCGATT37100.010.8948782
Produced by FastQC (version 0.11.5)