FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305947

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305947
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22704195
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG113800.055.301494
TAAGTCG32700.028.31498394
CAAGTCG87400.027.69450894
GAAGTCG127900.021.24002894
GTTCGAT33050.020.1936471
TTCGATT38350.016.0547562
AAAGTCG46650.014.40728894
TATACGG21750.014.04597734-35
TCGATTC41900.013.7971353
GTCCGAT16300.012.9754591
GTCGGAT27650.012.5786611
GTATACG22350.012.40715834-35
CGTATCG23800.012.0462183
GTATCGA34250.010.9781024
GTCGGTA15300.010.7516341
CTCGGTT33050.010.6656581
CGGAAAT46250.010.3654051
CGCGGAT24200.010.2933871
TTAGCGA31900.010.1661438
TAGCGAG33900.010.1209439
Produced by FastQC (version 0.11.5)