FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305948

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305948
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22704195
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG134400.047.2098294
CAAGTCG102400.026.85058494
TAAGTCG29750.025.2773194
GAAGTCG127750.021.04422694
CGCGTAT3157.779818E-917.904761
GTTCGAT30950.016.8562181
TTCGATT35200.014.6874992
TAGCGAG32700.013.0795119
TTAGCGA30450.012.9655178
GTCCGAT15050.012.8039861
CGCGTAA7601.8189894E-1212.368421
TATACGG21500.012.35116134-35
TCGATTC38150.012.1965923
CGCGGAT22600.012.0619471
CTCGGTT36250.011.6689651
AAAGTCG49800.011.51405694
TCGGATT29650.011.4131532
GACGCTA23200.011.3448284
CGTATCG20900.010.5693773
AATCGTA19000.010.3894735
Produced by FastQC (version 0.11.5)