FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305949

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305949
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21199609
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG195200.063.99897894
CAAGTCG143200.033.80586694
TAAGTCG55800.030.74372594
GAAGTCG185700.028.24555694
AAAGTCG64200.024.74454794
CGAAGTC86500.012.71445194
ACAAGTC126300.012.019793594
GCAAGTC128250.011.58050794
TATACGG16350.011.4984718
TATATCG9003.6379788E-1210.9666667
ATAAGTC57950.010.70578192-93
TTAAGTC71750.010.61184794
AGAAGTC245800.010.42107494
GTATACG16600.09.9096397
CGATAAT31050.09.3848644
GCGAAGT47650.08.8772394
TATGAGT58500.08.7572655
GTATAGA42350.08.6564348
TACGATT17400.08.64367994
TAACGCA21500.08.5255816
Produced by FastQC (version 0.11.5)